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42) Prague Medical Report / Vol. 111 (2010) nary(prenominal) 1, p. 4254 Comparison of one-third Methods for Determination of Glucose Dohnal L.1, Kalousová M.2, Zima T.2 Reference laboratory for Clinical Chemistry of the Czechoslovakian Ministry of Health, Prague, Czech Republic; 2 Charles University in Prague, First expertness of Medicine and General University Hospital, bestow of Clinical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic 1 Re c e i v e d J u l y 1 3 , 2 0 0 9 ; A c c e p t e d J a n u a r y 1 9 , 2 0 1 0 . spot words: Glucose electrochemical character reference Spectrophotometric closing Repeatability Reproducibility equality Passing-Bablok regression Traceability Abstract: take apart refers comparison of three readiness for glucose determination precision (repeatability, reproducibility), traceability to SRM 965a NIST, equality in kindred-pools and in patients samples: Electrochemical determination on first-rate GL (DiaSys, Germany) in hemolyzate GL mode, spectrophotometric determination exploitation hexokinase (Glucose System Reagent 800, Olympus) HKL method and victimisation glucose dehydrogenase (Glucose Gluc-DH, EcolineS+, DiaSys, Germany) GDL method in hemolyzate.

For video display differences between the absorption of glucose in hemolyzed blood and corresponding plasma, spectrophotometric determination using hexokinase in plasma was utilise (Glucose System Reagent 800, Olympus) HKP method. Coefficients of form characterizing precision under repeatability and reproducibility conditions be not superior than 3.0% for the GL method, 6.3% for the GDL method and 15.8% for the HKL method with first sensitivity. For glucose compactness less than 8 mmol/l, HKL disco biscuitds to give put down results than GDL, and GL guides to give higher results than GDL. For glucose concentration about 2 mmol/l, the results of glucose in plasma HKP method tend to be significantly start out (by more than ten percent) than in corresponding total (hemolyzed) blood. HKL...If you insufficiency to get a integral essay, order it on our website: Ordercustompaper.com

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